Review of the methods
- Tetsuo Yamaguchi, Daisuke Ono, and Ayami Sato (2020) Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA
- In: Yinduo Ji (ed.), Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Methods in Molecular Biology, vol. 2069, https://doi.org/10.1007/978-1-4939-9849-4_4
PCR-based methods
- Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. (2005)
Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 43(10):5026-5033
- Kondo Y, Ito T, Ma XX, Watanabe S, Kreiswirth BN, Etienne J et al. (2007)
Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother 51(1):264–274
- Milheirico C, Oliveira DC, de Lencastre H (2007)
Multiplex PCR strategy for subtyping the staphylococcal cassette chromosome mec type IV in methicillin-resistant Staphylococcus aureus: ‘SCCmec IV multiplex’. J Antimicrob Chemother 60(1):42-48
Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus. Antimicrob Agents Chemother 51(9):3374–3377
- Zhang K, McClure JA, Conly JM (2012)
Enhanced multiplex PCR assay for typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. Mol Cell Probes 26(5):218–221
WGS-based method
- SCCmec Finder
- Reference
- Kaya H, Hasman H, Larsen J, Stegger M, Johannesen TB, Allesoe RL et al (2018) SCCmecFinder, a web-based tool for typing of staphylococcal cassette chromosome mec in Staphylococcus aureus using whole-genome sequence data. mSphere 3(1). https://doi.org/10.1128/mSphere.00612-17